I am Dr.Parvathareddy sowmya. I completed my postgraduation in MD Pharmacology from Sree balaji medial college ,Chennai with Gold medal in MD Final examination,2017. In 2018 I joined SRIHER, as Senior Resident with a passion for learning, teaching and research in basic science. My areas of academic interests include pharmacovigilance , clinical trials and invitro cell line studies. I am the member of PTAMC committe .I got promoted as assistant professor in 2021 . I am currently assigned role of Pharmacology subject incharge for MBBS IInd year. Previously I was subject incharge for courses like Bsc Physiotherapy , Bsc Nursing, Msc neuroscience Bsc occupational therapy etc. I have been a speaker,moderator and judge in variousPharmacology postgraduate academic programmes.I was the organising secretary of ASPIRE 2024-Annual Rapid Review Course for MD Pharmacology postgraduates from across the country . . I was the Member – AITo infertility Committee . I have completed numerous courses like Competency Based Medical Education, conducted by MEU India and the Basic Course in Clinical Research conducted by ICMR. I have completed CISP III continous implementation sipport programme . I have been a Judge and Resource person at various national conferences.
Majority of the cardiovascular and renal diseases has been associated with Hypertension Primary hypertension also called as essential or idiopathic hypertension tends to be familial and the prevalence increases with age. Microalbuminuria has been correlated with microvascular damage in hypertension patients. To Compare the Efficacy of Losartan and Amlodipine in reducing microalbuminuria in patients with primary hypertension”. An open labeled parellelgroup cross sectioned study was conducted in SreeBalaji medical college and hospital,Department of General Medicine Chrompet during the period of March 2015 to December 2015.In our research study suggested that there are major differences between Angiotensin receptor blockers and calcium chaneel blockers in reducing microalbuminuria in essential hypertension. After 6-month of losartan treatment, thereduction in urine albumin level is statistically highly significant (p<0.01) 71.17±66.04**.compared with baseline101.95±94.70.
After 6 months of Amlodipine treatment group resulted in statistically not significant(p>0.05) in urine albumin excretion level 99.85±96.35compared with baseline 101.11±95.28 in essential hypertensive patients .Hence this study shows that losartan after administration for 6 months ,conferred significant advantages over amlodipine in terms of reduction of urinary albumin levelslevels in hypertensionpatients.
Background: Lung cancer is the most reported cancer worldwide and therapeutic resistance poses a major challenge. This in silico molecular docking and molecular dynamic simulation study was carried out to evaluate the binding affinity and stability of molecular interactions between the phytochemical Matairesinol and the molecular targets in Non-Small Cell Lung Carcinoma (NSCLC) which are implicated in the pathogenesis and development of therapeutic resistance, thereby exploring its potential anticancer activity in NSCLC.
Materials and Methods:
The three-dimensional structure of Matairesinol was obtained from the PubChem database.
The three-dimensional protein structures of target proteins were obtained from Protein Data Bank.
DOCK6 software package was used for the preparation of ligands and proteins. For the molecular dynamics study, the OPLS-2005 force field implemented in Desmond routine of Schrödinger software suite was used and the study was performed till 100 ns. Binding free energy (MM/GBSA), Root Mean Square Deviation (RMSD) and Root Mean Square Fluctuation (RMSF) values of all complexes were determined. Principal component analysis and Detailed Cross-Correlatio Maps were carried out.Results and Discussion: Matairesinol showed good binding energies and docking scores with all the targets tested in this study with relatively stable molecular interactions, with the highest affinity shown towards ALK. The interactions with EGFR wild type, KRAS G12C mutant protein, VEGFR2, C-MET and ALK were especially of high stability. Conclusion: The results of this study reveal the potential anti-cancer activity of Matairesinol in NSCLC with the possible added benefit of treating therapeutic resistance. Further studies are required to fully evaluate the target profile of Matairesinol in NSCLC and its therapeutic efficacy.
Keywords: Lung Cancer, Antineoplastic Agent, Non-Small Cell L
Background: Urticaria is a common skin disease which often causes impairment in the quality of life. The ideal drug for chronic urticarial would have antihistaminic and anti-inflammatory actions. Bepotastine besilate is a recently approved novel anti-allergic agent with multiple mechanisms of action; levocetirizine is a potent and selective second-generation H1 receptor antagonist used in the treatment of urticaria.
Aim: To compare the efficacy and safety of bepotastine besilate versus levocetirizine in patients with chronic spontaneous urticaria.
Methods: The study design is a randomised, open-label, parallel-group, prospective interventional study. The study subjects were randomly assigned to either of the two groups a and b, each group had 50 patients with chronic urticaria. Statistical analyses were performed using (SPSS, version 18) for all the variables. Chi-square test was used for comparison between categorical variables. An unpaired student’s t-test was done for quantitative variables.
Results: There was a significant decrease in mean urticaria activity score (P < 0.001), chronic urticaria quality of life (P < 0.001) and clinical global improvement (P < 0.001) in both the treatment groups but this improvement was higher in the bepotastine than in the levocetirizine group. There was no significant difference in the mean of absolute eosinophil count, C-reactive protein, aspartate transaminase, alanine transaminase from baseline to 4th week between the two study groups. Visual analogue scale showed statistically significant improvement from baseline to 4th week (P < 0.001) of follow-up but this increase was higher in levocetirizine group (0.64–4.24) than in bepotastine group (0.56–2.56)
Limitations: Blinding was not done. To assess the efficacy and safety of bepotastine, a larger study can be planned.
Conclusion: This study found that bepotastine is superior to levocetirizine and showed a statistically significant reduction in mean urticarial activity score 7, improved quality of life and clinical global improvement in patients with urticaria.
Title: Evaluation of Anti cancer effect of Bengalin, toxin from the black Indian scorpion venom in leukemia –An invitro study
INTRODUCTION:
Leukemia is a hematologic malignancy that arises from the dysfunctional proliferation of developing leukocytes. It is classified as either acute or chronic based on the rapidity of proliferation and as myelocytic or lymphocytic based on the cell of origin. Treatment depends on the type of leukemia but generally involves chemotherapy. Multiple genetic and environmental risk factors are identified in the development of leukemia. Based on previous studies, the global incidence of leukaemia is anticipated to increase to 311,594 fatalities and 474,519 newly diagnosed cases in 2020. In terms of newly diagnosed cases, leukaemia is anticipated to rank fifteenth, while it will account for 11th most cancer-related fatalities.
Scientific data has shown that scorpion toxin has several noteworthy medicinal qualities, such as the induction of cell cycle arrest, growth inhibition. It can be used to treat epilepsy, combat microbes, and block specific body channels. More specifically, the active peptide bengalin has been found to have an impact on apoptotic and cell death pathways, which could potentially open up new possibilities for alternative therapeutic applications.
Prior research has demonstrated that the suppression of U937 cell growth was achieved through apoptosis, as indicated by the presence of damaged nuclei, cell cycle arrest in the sub G1 phase, an increase in early apoptotic cells, an enhancement of DNA fragmentation, and a decrease in telomerase activity.Further analysis indicates that the BAX:BCL2 ratio increased following bengalin treatment. In addition, bengalin caused a decrease in the mitochondrial membrane potential (MMP) leading to the release of the cytochrome c into the cytosol. This in turn results in the release of heat shock proteins (HSP) 70 and 90 and thereby activation of the caspase-9 and caspase-3 enzymes and induction of the poly (ADP-ribose) polymerase (PARP) cleavage.
Given the scarcity of research pertaining to this specific subject matter and the global prevalence of leukaemia, which has exhibited a notable upward trend in recent years.
AIM:
To elucidate the anti cancer potential of the bengalin toxin on leukaemia using in-vitro western blot assay on cell lines U937
OBJECTIVES:
METHODOLOGY:
Bengalin are purified peptide obtained from the Indian black scorpion venom extracted using ion exchange chromatography, HPLC, MTT and apoptotic assay.
TYPE OF STUDY AND STUDY DESIGN: In-vitro study
PLACE OF STUDY: Sri Ramachandra Medical College and Research Institute
Study cell line: U937
PROCEDURE:
Acquiring the Bengalin Toxin:
Venom was collected from live animals as done previously, then dissolved in 0.02 M phosphate buffer with a pH of 7.2, followed by centrifugation at 10,000 × g for 15 minutes to remove mucous and undissolved matter. The venom proteins were separated using a DEAE (diethyl aminoethyl) cellulose ion-exchange chromatography column, eluted with 0.02 M phosphate buffer at pH 7.2 along with gradual NaCl additions. HPLC with a Waters HPLC Protein Pak 300SW column and 10 mM sodium phosphate buffer containing 0.1 M NaCl (pH 7) at a flow rate of 0.8 ml/min was employed to further purify the cytotoxic protein fraction, which is estimated using Bradford’s method. The homogeneity of the protein fraction was determined by running a 12% SDS PAGE gel at 100 V and 40 mA for 1.5 hours at 4 °C, followed by staining with Coomassie brilliant blue to visualize protein bands.
Measurement of the concentration of Caspases:
The caspase-8, 9 and 3 activity is estimated by colorimetric assay kits. Caspase-8, 9 and 3 are measured by the production of p-nitroaniline.
MTT assay
In 96-well plates containing a final volume of 100 µl/well, prepare cells and test compounds according to the MTT Assay Protocol. The recommended duration of exposure is incubated. To reach a final concentration of 0.45 mg/ml, add 10 µl MTT Solution per well. Incubate at 37°C for one to four hours. Unify formazan crystals in each well by adding 100 µl of soluble solution. Stir to achieve thorough solubilization. Absorbance is measured at 570 nm.
Apoptotic Assay
Healthy cells are identified as those negative for both propidium iodide (PI) and Annexin V. Cells that are negative for PI but positive for Annexin V are classified as apoptotic. Cells positive for both PI and Annexin V are categorized as necrotic.
Awarded 1 st prize in Tamcon 19 – 3 rd October 2019,In Tagore Medical College
Won Best poster in poster competition in nutrition in ancient epics and puranas of india event as a part of National Nutrition Month celebration
Member –Pharmacy therapeutic Antibiotic Monitoring Committee, Sri Ramachandra Medical Centre
This committee monitors the usage of oral and parenteral antibiotics and ensures that the DDD is within normal limits. It monitors the usage of restricted antibiotics.
This committe monitors and develop the cases regarding the core subjects in MBBS as a part of an integrated learning