RADIATION BIOLOGY LAB-
Our lab deals with various aspects of radiation biology which includes radiation biodosimetry, biological impacts of low dose radiation and non- targeted effects and high LET in clinical radiation therapy, adopting cytogenetic and molecular markers using in-vitro (cell lines) and in-vivo (human participants) models. We also actively participate in inter-laboratory comparisons and triage with collaborating institutions (BARC, Mumbai, IGCAR, Kalpakkam, INMAS, New Delhi, RENEB, Europe and Columbia University, USA) for improving the rising demands that can occur during radiation fallout.
The lab was formerly the only non-DAE laboratory in India that was accredited by AERB and now it is accredited by NABL.
TEAM
Current candidates
1. Aishwarya S, PhD Research Scholar
“Validation of mini Dicentric chromosome aberration as cost-effective and Quantification of Gamma H2AX using ELISA as rapid marker for radiation biodosimetry”
2.Aishwarya T A, PhD Research Scholar
“Studies on Radiation-Induced Adaptive Response and its implications on Cancer Radiation Therapy”
3.Divya K Mohan, PhD Research Scholar
“Assessment of Geometrical Dose Distribution, DNA Damage and Stochastic Risk Prediction in Pediatric subjects undergoing Computed Tomography”
4.Nandhini K, PhD Research Scholar
“Studies on Gene Expression Biomarker for Rapid Triage of Radiation Exposed Population”
5.Rahul Kabir, PhD Research Scholar
“Chromosomal Radiation Sensitivity in directly Irradiated and Bystander Normal and Cancer cells exposed to Low and High Linear Energy Transfer Radiations”
PROJECTS-
Ongoing projects
- BRNS, 2024- 2027 (3 years)
“Chromosomal Sensitivity in directly Irradiated and Bystander Normal and Cancer cells after Low and High LET Irradiation with implications for prospective therapy induced delayed health effects”
- SPARC, 2024- 2026 (2 years)
“A Tiered Biodosimetry Approach for management of Large Scale Radiological and Nuclear Emergencies”
Completed Projects
- AERB, INDIA, 2021-2023 (2 years), 35 lakhs
“Establishment and capacity building for rapid radiation triage and dose estimation using gene expression biomarker”
- IGCAR, DAE, INDIA, 2017-2020 (3 years), 24.98 lakh
“Standardization and testing of Premature Chromosome Condensation (PCC) assay for radiation triage and dose estimation”
- AERB, INDIA, October 2014- 2017 (3 years), 50.85 lakhs
“Biological effects of low dose alpha particle radiation exposure to blood lymphocytes for biodosimetry”
- SERB, DST, Govt. of India, 2013- 2016 (3 years), 46.23 lakhs
“Biological effects of low dose ionizing radiations received during diagnostic radiological procedures”
- LSRB, DRDO, Govt. of India, 2013- 2015 (2 years), 24.9 lakhs
“Standardization and establishment of -H2AX assay for triage biodosimetry”
- Institute of Nuclear Medicine & Allied Science, DRDO, Govt. of India, 2011- 2013, (2 years), 14.9 lakhs
“Inter laboratory comparison of radiation dose response calibration curve using dicentric assay”
- ICMR, India, 2010- 2013 (3 years), 20.9 lakhs
“Cytogenetics of idiopathic mental retardation”
- DST, India, 2007- 2010 (3 years), 22 lakhs
“Genomic instability in Bystander Human Cells and their Progeny exposed to Ionizing Radiation using 3-D cultures”
- DST, INDIA, 2006- 2009 (3 years), 10 lakhs
“Study on the genetic variation of HLA-G alleles in couples with unexplained recurrent spontaneous abortion in south India”
- AERB, INDIA, 2002- 2005 (3 years) 18.5 lakhs
“Biological Dosimetry Preparedness- Validation on the measurement of translocation frequency by chromosome painting and G-banding for dosimetry”
PUBLICATIONS-
- Hariharan S, Seethashankar S, Kannan N, et al. Enhanced Γ-H2AX foci frequency and altered gene expression in participants exposed to ionizing radiation during I-131 nuclear medicine procedures. Nuclear Medicine and Molecular Imaging. Published online July 12, 2024. doi:10.1007/s13139-024-00872-3.
- Selvan GT, Venkatachalam P. Ataxia telengectesia protein influences Bleomycin-Induced DNA damage in human fibroblast cells. Cell Biochemistry and Biophysics. Published online May 2, 2024. doi:10.1007/s12013-024-01275-z
https://pubmed.ncbi.nlm.nih.gov/38696104/
- Kannan N, Koshy T, Raavi V, et al. Candidate Gene Expression in Regional Population and Its Relevance for Radiation Triage. Cytogenet Genome Res. 2023;163(3-4):210-222. doi:10.1159/000531258
https://pubmed.ncbi.nlm.nih.gov/37253332/
- Ananthanarayanan AT, Raavi V, Kondaveeti SS, Ramachandran I, Perumal V. Insights on the Radiation-Induced adaptive response at the cellular level and its implications in cancer therapy. Cytogenetic and Genome Research. Published online October 31, 2023:1-17. doi:10.1159/000534500
https://pubmed.ncbi.nlm.nih.gov/37906989/
- Gonon G, De Toledo SM, Perumal V, Jay-Gerin JP, Azzam EI. Impact of the redox environment on propagation of radiation bystander effects: The modulating effect of oxidative metabolism and oxygen partial pressure. Mutation Research Genetic Toxicology and Environmental Mutagenesis. 2022;883-884:503559. doi:10.1016/j.mrgentox.2022.503559
https://pubmed.ncbi.nlm.nih.gov/36462795/
Book chapter –
- Low Dose Radiation in Health and Disease, Chapter 6: Low Dose Radiation and Risk for Non- Malignant Disease, Narosa Publishing House, 2024
Yasheswinee R, Vimal Anand M, Divya K Mohan and Venkatachalam P
